Tegrative modeling can contribute a whole lot to solving this problem. We propose that members of your smFRET neighborhood that are serious about working with smFRET for integrative structural biology take part in the CASP competitors. Involvement could progress in a number of stages: (1) Predicting single- and multi-state structural models: the smFRET neighborhood will only submit distances that could be evaluated with respect to the identified (but undisclosed) crystal structure(s). (two) Predicting ensembles as inside the case of CBM56: for targets which are identified as complicated by the predictors and for which several achievable folds are submitted without a clear winner, a FRET-assisted round may very well be insightful where the FRET distances distributions can be made use of as an experimental `ground truth’ for checking whether numerous conformations in an exchange are present. These suggestions apply largely to present and future practitioners of smFRET-driven integrative modeling. That getting mentioned, smFRET is one of several biophysical tactics that may give experimental restraints in integrative modeling (XL-MS, single-particle cryoEM, NMR, SAXS). Consequently, we propose that, at a later stage, an all-biophysics integrative structural biology competitors be established.SmFRET meetingsSeveral gatherings of FRET practitioners in the Annual Biophysical Society Meetings, supported by the Biological Fluorescence subgroup, provided a platform for arranging future activities and establishing the FRET neighborhood. As additional joint actions, satellite HDAC4 drug meetings to the Conference on Methods and Applications in Fluorescence (MAF) happen to be organized to discuss practices, standards, competitions, and joint publications. We envision an occasional devoted meeting for the smFRET community, for instance the Bunsen meetings on FRET held in 2011 and the international discussion meeting in 2016 at the Max Planck Institute for Biophysical Chemistry in Gottingen, Germany (http:// fret.uni-duesseldorf.de/cms/home.html). Nonetheless, to open these meetings to smFRET practitioners outdoors of Europe, we propose to rotate the venue amongst continents. We also suggest working with the satellite meetings and workshops to disseminate details (information of accurate FRET measurements, frequent practices, requirements and competitions) and to offer newcomers the likelihood to interact using the experienced researchers in the field.Lerner, Barth, Hendrix, et al. eLife 2021;10:e60416. DOI: https://doi.org/10.7554/eLife.35 ofReview ArticleBiochemistry and Chemical Biology Structural Biology and Molecular BiophysicsInspired by the on-line seminars emerging in response to the COVID-19 pandemic, smFRET webinars and net conferences open to all should be pursued. They give FRET researchers the unique chance to attend and socialize virtually and would be a forum for great scientific practice of open science for the FRET community.Particular issues in journalsTo additional stimulate newcomers to engage in advanced smFRET experiments, the FRET community could benefit by hosting particular concerns in Caspase 9 Species journals dedicated to data analyses (e.g., Information in Brief, Techniques in Molecular Biology, or Nature Protocols). Here, several laboratories can describe typical datasets or protocols for the procedures they’ve developed. Also, journals disseminating methodologies and protocols from A-to-Z by means of video recordings might be valuable. For example, there’s a unique problem focusing on FRET planned in the Journal of Visualized Experiments (https://www.jove.com/ strategies.