group than in the T0 group. Adding curcumin in eating plan drastically decreased TBIL level (p = 0.043) in the T500 + AFB1 group with respect towards the T0 + AFB1 group. As expected, there was no important distinction in TBIL level involving the T500 + AFB1 group and T0 group (p 0.05) (Figure 1E). No important distinction in ALP (p = 0.621) plus a decreasing trend in ALP (p = 0.676) had been GLUT4 Storage & Stability observed amongst groups (Figure 1F). There was no significant enhance in ALT (p = 0.246) and AST (p = 0.065) activity within the T0 + AFB1 group relative to these inside the T0 group. Adding curcumin into eating plan inhibited the activities of ALT (p = 0.544) and AST (p = 0.140) inside the T500 + AFB1 group relative to those inside the T0 + AFB1 group, but with no substantial variations. No important distinction in ALT and AST activity between the T0 + AFB1 group plus the T0 group was identified (p 0.05) (Figure 1G,H). three.2. Evaluation of Pathological Sections and Ultrastructural Assessment in Liver Histopathological examination of H E-stained livers shown in Figure two. Inside the T0 group, hepatocytes morphology was typical (Figure 2A). AFB1 administration brought on clear toxicity containing vacuolation of hepatocytes, KDM3 Accession swelling of hepatocytes, and inflammatory cell infiltration in the T0 + AFB1 group in comparison with the T0 group (Figure 2B). Dietary curcumin protected the liver against harm via the lower within the quantity of inflammatory cells and swelling of hepatocytes in the liver of ducks inside the T500 + AFB1 group compared with inside the T0 + AFB1 group (Figure 2C). Some inflammatory cells and swelling of hepatocytes in the T500 + AFB1 group compared with the T0 group was noticed. The results of this study demonstrate that dietary curcumin could guard duck liver against acute damage induced by AFB1 administration. The liver ultrastructure is shown in Figure 2. Within the T0 group, the cell nucleus and mitochondrial ridge of hepatocytes had been clearly visible along with the chromatin inside the cell nucleus was evenly distributed (Figure 2D). In comparison using the T0 group, the hepatocyte nucleus was visibly deformed; chromatin was aggregated and also the hepatocyte mitochondrial ridge was enlarged and deformed within the T0 + AFB1 group (Figure 2E). As anticipated, in comparison with all the T0 + AFB1 group, hepatocyte nucleus and mitochondrial ridge were clearly visible as well as the chromatin aggregation of hepatocytes was observed inside the T500 + AFB1 group (Figure 2F). Additionally,Foods 2021, ten,five ofFoods 2021, 10, x FOR PEER Critique the5 the hepatocyte nucleus and mitochondrial ridge were clearly visible when comparing of 19 T500 + AFB1 group and T0 group.Figure 1. The plasma biochemical levels of ducks exposed to AFB1 at 12 h. (A) The TP content in the Figure 1. The plasma biochemical levels of ducks exposed to AFB1 at 12 h. (A) The TP content inside the plasmaof ducks; (B) The ALB content material inin the plasma of ducks; (C) The GLO contentthe the plasma plasma of ducks; (B) The ALB content material the plasma of ducks; (C) The GLO content in in plasma of of ducks; (D) The price of ALB/GLO; (E) The TBIL activity inside the plasma of ducks; (F) The ALP acducks; (D) The rate of ALB/GLO; (E) The TBIL activity in the plasma of ducks; (F) The ALP activity tivity in the plasma of ducks; (G) The ALT activity inside the plasma of ducks; (H) The AST activity in within the plasma of ducks; (G) The ALT activity inside the plasma of ducks; (H) The AST activity within the the plasma of ducks; (I) The rate of AST/ALT. Values imply the mean SEM (normal error (SE) of Foods 2021,