Roteins with direct or indirect association with cell junctions and/or the cytoskeleton (Table 1); (ii) seven Sudan IV Epigenetic Reader Domain proteins from the secretory pathway; (iii) 4 mitochondrial proteins; (iv) two chaperone proteins; and (v) one nucleuscytoplasm shuttling protein. Two proteins from the mitochondrion group and one protein from the secretory pathway happen to be detected at the plasma membrane. Thus, 15 out of 26 proteins could be directly or indirectly associated with all the plasma membrane (12 from cell junctions or the cytoskeleton, two from the mitochondrial group, and one from the secretory group). A common subcellular classification from the 26 proteins is illustrated in Figure 1A.Int. J. Mol. Sci. 2018, 19,3 ofInt. J. Mol. Sci. 2018, 19, x FOR PEER REVIEW3 ofFigure 1. (A) Venn diagram representing the distribution of 26 candidate proteins to interact with Figure 1. (A) Venn diagram representing the distribution of 26 candidate proteins to interact with CX26. CX26. The subcellular classification and the quantity of proteins in every with the 5 distinct groups are The subcellular classification and also the quantity of proteins in each of your five distinct groups are indicated indicated too as their overlapping sorting; (B) The 2′-Deoxyadenosine-5′-monophosphate supplier network of proteinprotein interaction (PPI) is also as their overlapping sorting; (B) The network of proteinprotein interaction (PPI) is predicted predicted at http://stringdb.org. Acronyms are based on the genes listed on Table 1 in addition to at http://stringdb.org. Acronyms are in accordance with the genes listed on Table 1 along with actin B actin B (ACTB), (TUBA1A), and arginine succinate synthase 1 (ASS1). Black 1 (ASS1). Black circles (ACTB), tubulin tubulin (TUBA1A), and arginine succinate synthase circles represent proteins represent proteins absent in the list of interactors fed by the user to the computer software input list. Asterisks absent in the list of interactors fed by the user for the software input list. Asterisks indicate protein indicate protein interactions manually added to the PPI network based on the literature (MAP7, interactions manually added for the PPI network in line with the literature (MAP7, RAI14, Homer2). RAI14, Homer2). Striped circles denote the 4 CX26 interactor candidates previously demonstrated Striped circles denote the four CX26 interactor candidates previously demonstrated to associate with to associate with other CX. Thicker lines indicate experimentally demonstrated associations that may other CX. Thicker lines indicate experimentally demonstrated associations that may possibly be either extra be either much more direct interaction. A dashed line indicates dashed of an intermediate interactor. reproducible or possibly a reproducible or a direct interaction.
The PPI network contains all 12 proteins classified as cell junction/cytoskeleton (Table 1, Figure 1B) and one particular in the mitochondrion group (argininosuccinate synthase 1ASS1, Figure 1A), which has been reported to interact with the plasma membrane inInt. J. Mol. Sci. 2018, 19, 2535 Int. J. Mol. Sci. 2018, 19, x FOR PEER REVIEW4 of 20 4 ofaddition towards the mitochondrial outer membrane [28]. Within the present study, we report the evaluation of membrane [28]. Within the present study, we report the analysis from the group of 13 proteins present in the the group of 13 proteins present inside the PPI network. PPI network.2.2. Recognized CX Binding Partners within the CX26 Molecular Complicated 2.two. Known CX Binding Partners in.