Iral ligament, spiral limbus, spiral limbus, and external sulcus cells (Figure five). DAAM1 is also present in spiral ganglion cells and external sulcus cells (Figure 5). DAAM1 can also be present in spiral ganglion cells (Figure five). Likewise, (Figure 5). Likewise, in stria vascularis DAAM1, staining Enduracidin B Anti-infection appeared a lot more evident than CGN and FLNB in stria vascularis DAAM1, staining appeared additional evident than CGN and FLNB staining. On P14, staining. On P14, CX26 distributed to the OC, stria vascularis, spiral ligament, spiral limbus, and also the CX26 distributed towards the OC, stria vascularis, spiral ligament, spiral limbus, plus the spiral ganglion. spiral ganglion. The tectorial membrane is acellular and known to yield unspecific staining on account of The tectorial membrane is acellular and known to yield unspecific staining due to spontaneous spontaneous fluorescence. CX26 and all 3 adaptor proteins look to become coexpressed within the OC fluorescence. CX26 and all three adaptor proteins appear to be coexpressed in the OC despite the fact that OC even though OC cell identity could not be precisely defined given that distinct cell variety markers haven’t cell identity could not be precisely defined considering that distinct cell type markers haven’t been employed. been employed.Figure four. Indirect immunofluorescence of adult (P60) mouse liver cryosections with antiCx26 Figure 4. Indirect immunofluorescence of adult (P60) mouse liver cryosections with antiCx26 antibody antibody (green) and CGN, FLNB, and DAAM1 antibodies (all in red). DNA is in a white pseudo (green) and CGN, FLNB, and DAAM1 antibodies (all in red). DNA is inside a white pseudo color, colour, as outlined by DAPI staining. The analysis was performed at confocal microscopy with zsections based on DAPI staining. The evaluation was performed at confocal microscopy with zsections of of 0.five m (LSM880, Carl Zeiss, Oberkochen, Germany). Each and every image consists of your maximum intensity 0.5 (LSM880, Carl Zeiss, Oberkochen, Germany). Each image consists of your maximum intensity projection of all zsections obtained. Arrowheads: signal in the plasma membrane. Arrows: merged projection of all zsections obtained. Arrowheads: signal in the plasma membrane. Arrows: merged signals. Scale bar: ten m. signals. Scale bar: ten .Int. J. Mol. Sci. 2018, 19,eight ofInt. J. Mol. Sci. 2018, 19, x FOR PEER REVIEW8 ofFigure 5. Indirect immunofluorescence of P14 mouse cochlea cryosections and zoomingin at the Figure 5. Indirect immunofluorescence of P14 mouse cochlea cryosections and zoomingin in the organ organ of Corti (OC) indicated by a rectangle. The sections highlight the spiral ligament (SL), stria of Corti (OC) indicated by a rectangle. The sections highlight the spiral ligament (SL), stria vascularis vascularis (SV), spiral limbus (SLm), spiral ganglion (SG), external sulcus cells (ES), OC, and tectorial (SV), spiral limbus (SLm), spiral ganglion (SG), external sulcus cells (ES), OC, and tectorial membrane membrane (TM). Labeling by antiCx26 antibody (green) and antibodies for CGN, FLNB, and (TM). Labeling by antiCx26 antibody (green) and antibodies for CGN, FLNB, and DAAM1 (all in DAAM1 (all in red) are presented. DNA is in a white pseudo color, based on DAPI staining. The red) are presented. DNA is within a white pseudo colour, as outlined by DAPI staining. The evaluation was analysis was performed at confocal microscopy with zsections of 1 m (LSM880, Carl Zeiss, perform.