And 0.05 glutaraldehyde in PBS (two min), neutralized by 0.1 M glycine/PBS (five min),
And 0.05 glutaraldehyde in PBS (two min), neutralized by 0.1 M glycine/PBS (five min), and after that blocked in three BSA/PBS (30 min). Noninvasive parasites or egressed vacuoles were stained…