L of Chinese Medicine 2018, 13(Suppl 1):72 Background: MYCs are a essential transcription factors within the JA signaling pathway and are important members on the family of bHLH transcription aspects. Preceding studies have shown that MYC2 can be a essential constructive regulator within the MYCs family and plays a crucial part in JA, ABA signaling pathway and has positive effects around the biosynthesis of flavonoids (such as anthocyanins). Salvia miltiorrhiza Bunge is AMAS MedChemExpress actually a sort of regular Chinese medicine, its roots and stems possess a high medicinal worth which can which plays a vital role in curing cerebrovascular diseases, irregular period, blood circulation and anti-cancer. The active ingredient of S. miltiorrhiza is mostly hydrosoluble phenolic acids and liposoluble tanshinone. On the other hand, the effects of MYC2 on the secondary metabolism of S. miltiorrhiza is seldom known. Benefits: We firstly introduced the transcription element AtMYC2 from Arabidopsis thaliana into S. miltiorrhiza hairy roots, the outcomes showed that AtMYC2 couldn’t only increase the tanshinones content, but additionally increase the salvanic acid content. Subsequently, depending on the sequence of AtMYC2, we cloned a sequence from S. miltiorrhiza which was higher homology with AtMYC2 and named it SmMYC2. Then we constructed the SmMYC2 overexpressing vector and obtained the transgenic hairy roots. The outcomes showed that SmMYC2 could substantially enhance the content material of tanshinone compared with the manage group. Conclusion: Overexpression of AtMYC2 and SmMYC2 can significantly induce the accumulation of active compounds in S. miltiorrhiza which indicates that MYC2 play a positive part within the secondary metabolism of Salvia miltiorrhiza. Keyword phrases: Salvia miltiorrhiza, tanshinones, Salvanic acid, MYC2, secondary metabolism. 73 Assessment with the inhibition possible of Gambogenic acid against human UDPglucuronosyltransferases Xiaoya Sun1,two, Shiyang Li2, Xia Lv2, Hui Tang1, Guangbo Ge2 Key Laboratory of Xinjiang Phytomedicine Resource and Pathway Inhibitors MedChemExpress Utilization, Ministry of Education, Pharmacy College of Shihezi, Xinjiang 832000, China; 2Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China Correspondence: Hui Tang [email protected]; Guangbo Ge [email protected] Journal of Chinese Medicine 2018, 13(Suppl 1):73 Background: Gambogenic acid (GNA) is amongst the main bioactive compounds in Gamboge. Lately, increasing evidence has indicated that GNA exerts promising anti-tumor effects [1]. Prior LC-UV fingerprint and UGT1A1 inhibition profile research have indicated that the extract of gamboge exhibited evident inhibitory effects against UGT1A1 in human liver microsomes (HLMs) and GNA showed most potent inhibition on UGT1A1. Nonetheless, there is absolutely no reportedFig. 1 Assessment in the inhibition prospective of GNA towards human UGTsevidence at the moment around the inhibitory effects of GNA against prevalent phase II drug metabolizing enzymes. Within this study, we investigated GNA inhibition against 4-methylumbelliferone (4-MU) glucuronidation and N-3-carboxypropyl-4-hydroxy-1,8-naphthalimide(NCHN) glucuronidation. Components and solutions: Recombinant UGTs-catalyzed 4-MU-glucuronidation reaction was preliminary employed to evaluate the inhibition potential of GNA towards various UGTs. Meanwhile, a novel applicable ratiometric fluorescent probe for highly selective sensing the enzyme activity of UGT1A1 [2], NCHN, was utilized to figure out the inhibitory effects.