Of the mechanical properties gives novel data to tune and modify the synthesis procedure for realizing more robust, durable and steady soot particle films, as needed for the aforementioned applications. Consequently, an experimental investigation of the mechanical properties of flame formed soot nanoparticles collected as nanostructured films could be a helpful addition to the literature, also as a piece of perform of excellent relevance from a material science point of view. Quite a few wellestablished strategies exist on the macroscale and around the microscale to characterize the mechanical behavior of a offered material. Especially, the indentation strategy permits measuring the mechanical properties by indenting the material, i.e., by pressing a probe at a defined force on the sample surface so as to deform it. Procedures with nanometric resolution are necessary to characterize and test nanosized and nanostructured supplies [31]. To this aim, nanoindentation characterizations based on Atomic Force Microscopy (AFM) are becoming increasingly attractive. The most critical advantages of AFM nanoindentation are the measurement of mechanical properties simultaneously with surface topography, the distinctive force sensitivity of the approach (down to nNewton) and the probe size within the order of nanometers, which are important to execute indentation and molecular pulling experiments in the nanoscale [3234]. Within this paper, an experimental investigation of nanomechanical properties of flame formed carbonaceous particles has been performed for the very first time by signifies of AFM nanoindentation. The approach and the experimental protocol had been first finetuned and implemented by analyzing the different plastic behavior of reference materials, e.g., polyethylene naphthalate and extremely oriented pyrolytic graphite. Two distinct classes of soot particles had been produced and thermophoretically collected from ethyleneair laminar premixed flames and preliminary characterized in terms of hardness, H, and Young’s modulus, E. This work represents a initial attempt to Recombinant?Proteins Neurofilament light polypeptide/NEFL overcome a lack of experimental details about the mechanical properties of soot layers and to furnish direct experimental measurements of hardness and elastic modulus of nanostructured films of flameformed carbon particles. two. Materials and Approaches Two distinctive laminar premixed flames of ethylene and air operated at atmospheric pressure were used to generate films of carbon nanoparticles. The chosen flame situations along with the sampling position are reported in Table 1. The flames have been stabilized on a watercooled McKenna burner, along with the flame equivalence ratio was changed in order to create particles with diverse dimension, nanostructure and graphitization degree.Appl. Sci. 2021, 11,3 ofParticles had been collected at a fixed sampling position, equal to 14 mm from the burner surface, working with a thermophoretic sampling technique. The technique is created of a doubleacting pneumatic cylinder equipped with a substrate holder mounted over a mobile extension. Particles were collected by thermophoresis, due to the temperature gradient generated between the hot gases along with the cold substrate. The residence time with the substrate in flame was optimized and kept constant at one hundred ms, when the number of insertions was varied based on.