As funded by DGAPA-UNAM via PAPIIT Grant No. IN211020 to G.
As funded by DGAPA-UNAM by means of PAPIIT Grant No. IN211020 to G.S.R. On top of that, the project was accomplished thanks to the scholarships Num 443241 to R.A. and 958307 to A.L. supported by Consejo Nacional de Ciencia y Tecnolog (CONACYT), M ico. Institutional Evaluation Board Statement: Not applicable.Molecules 2021, 26,19 ofInformed Consent Statement: Not applicable. Information Availability Statement: Not applicable. Acknowledgments: The authors are indebted to Jorge Arturo Ya z and Paul Gaytan for oligonucleotide synthesis and DNA sequencing, Jerome Verleyen, for laptop cluster supervision, to Juan Manuel Hurtado-Ram ez and David Santiago Casta da-Carre for pc technical support, at the same time as Shirley Ainsworth and Omar Arriaga, for librarian assistance. Conflicts of Interest: The authors declare that they have no conflict of interest. Samples Availability: Samples on the plasmids are Gisadenafil Autophagy accessible in the authors.
moleculesArticleCell Culture Characterization of Prooxidative Chain-Transfer Agents as Novel Cytostatic DrugsVictoria Heymans 1 , Sascha Kunath 1 , Parvana Hajieva 2 and Bernd Moosmann 1, Evolutionary Biochemistry and Redox Medicine, Institute for Pathobiochemistry, University Medical Center of the Johannes Gutenberg University, 55128 Mainz, Germany; [email protected] (V.H.); [email protected] (S.K.) Institute for Translational Medicine, MSH Medical College Hamburg, 20457 Hamburg, Germany; [email protected] Correspondence: [email protected]; Tel.: +49-6131-39-Citation: Heymans, V.; Kunath, S.; Hajieva, P.; Moosmann, B. Cell Culture Characterization of Prooxidative Chain-Transfer Agents as Novel Cytostatic Drugs. Molecules 2021, 26, 6743. https://doi.org/ ten.3390/molecules26216743 Academic Editors: Visnja Stepanic and Marta Ku erovChlupovc c Received: eight October 2021 Accepted: 4 November 2021 Published: eight NovemberAbstract: Prooxidative therapy is often a well-established concept in infectiology and parasitology, in which prooxidative drugs like artemisinin and metronidazole play a pivotal clinical role. Theoretical considerations and earlier studies have Gamma-glutamylcysteine Technical Information indicated that prooxidative therapy could possibly also represent a promising method in oncology. Right here, we’ve investigated a novel class of prooxidative drugs, namely chain-transfer agents, as cytostatic agents in a series of human tumor cell lines in vitro. We’ve got found that distinct chain-transfer agents of your lipophilic thiol class (like dodecane-1-thiol) elicited half-maximal effective concentrations in the low micromolar range in SY5Y cells (human neuroblastoma), Hela cells (human cervical carcinoma), HEK293 cells (immortalized human kidney), MCF7 cells (human breast carcinoma), and C2C12 cells (mouse myoblast). In contrast, HepG2 cells (human hepatocellular carcinoma) have been resistant to toxicity, presumably through their higher detoxification capacity for thiol groups. Cytotoxicity was undiminished by hypoxic culture circumstances, but substantially lowered following cellular differentiation. In comparison to 4 disparate, clinically used reference compounds in vitro (doxorubicin, actinomycin D, 5-fluorouracil, and hydroxyurea), chaintransfer agents emerged as comparably potent on a molar basis and on a maximum-effect basis. Our final results indicate that chain-transfer agents possess a promising baseline profile as cytostatic drugs and should be explored additional for anti-tumor chemotherapy. Keyword phrases: chain-transfer agent; chemotherapy; free of charge radical chain reaction; lipid.