Of IA in human, its acute toxicity was performed in mice. Outcomes reveals that IA showed high antilymphoma activity and BSL with an EC50 of two.4 mg/kg and LC50 16.7 /mL, respectively. The molecular docking study revealed that IA has strong interaction on all targets employed. Inside the acute oral toxicity, IA had a LD50 of 149 mg/kg. The results showed that the activities of IA such as antilymphoma activity, BSL, acute toxicity, and in silico interactions were close for the methotrexate, an anticancer drug employed as constructive control. These findings suggest that IA may perhaps serve as a candidate for the development of a new drug to combat lymphoma. Keywords: incomptine A; antilymphoma activity; molecular docking; acute toxicity; brine shrimp lethality; cancer; non-Hodgkin lymphomaPublisher’s Note: MDPI stays neutral with regard to 5-Azacytidine Bacterial jurisdictional claims in published maps and institutional affiliations.1. Introduction Sesquiterpene lactones (SLs) are a group of phytochemicals present in species on the plant kingdom, particularly Compstatin site abundant in the family Asteraceae with greater than 5000 distinct structures reported. They may be a sizable group of low molecular weight specialized metabolites using a 15 carbon skeleton. SLs are thinking of as a class of organic compounds with pharmaceutical relevance that may be an alternative for cancer therapy [1,2]. SLs also have a wide variety of biological activities which include antiamoebic, antigiardial, trypanocidal, antidiabetic, antibacterial, cytotoxic, antitumor, and phytotoxic [3]. In the case of antitumor activity, studies on their molecular mechanism action have reported that SLs exercise their antitumor properties by means of a change within the redox cellCopyright: 2021 by the authors. Licensee MDPI, Basel, Switzerland. This short article is an open access article distributed beneath the terms and circumstances on the Inventive Commons Attribution (CC BY) license (licenses/by/ four.0/).Molecules 2021, 26, 6646. ten.3390/moleculesmdpi/journal/moleculesMolecules 2021, 26,2 ofbalance and their impact on different signaling pathways like the nuclear factor-kB (NF-kB) pathway and signal transducer and activator of transcription 3 (STAT3). These properties cause affectation within the cell cycle, boost apoptotic variables, reduce cellular invasion and metastasis things, decrease the anti-apoptotic factors, and enhance the production of free of charge radicals related together with the induction of apoptosis [1,2]. In vivo antitumor activity of numerous SLs for example parthenin, arteminolides A , eupatoriopicrin, and parthenolide (Pa) has been reported [8]. Non-Hodgkin lymphomas (NHLs) are a heterogenous group of neoplasms or lymphoproliferative malignancies, derived in the standard cells of lymphoid tissue including lymphocyte and histiocytes in any of their developmental stages. In 2015, almost 4.3 million persons were reported to have non-Hodgkin lymphoma (NHL), which accounted for 231,400 deaths around the globe, predominantly in adults [9,10]. In the case of Mexico till 2016, NHL was the fourth most common result in of cancer [11]. Quite a few drugs are obtainable for its therapy which includes cyclophosphamide, prednisone, cisplatin, fludarabine, methotrexate (MTX), doxorubicin, vincristine, and etoposide. However, moreover to the therapeutic properties, the majority of these drugs showed limited chemotherapeutic efficacy and have many unwanted effects [12]. Previously, the fractionation from a CH2 Cl2 extract of the leaves of Decachaeta incompta (DC.) R. M. King H. Rob led to t.