And that this influences the expression of proteins that 2. Results and Discussion are involved in cell adhesion and cell mobility. 2. Results and Discussion2.1.2.1. Impact TNF onon ProteoglycanTranscription and Expressionin HMVECs Impact of of TNF Proteoglycan Transcription and Expression in HMVECsTNF was added to the cell culture medium in an effort to screen for all round TNF induced two.1. Effect of added to the cell culture medium in order HMVECs TNF wasTNF on Proteoglycan Transcription and Expression in to screen for overall TNF induced changes and changeschemokine GAGGAG co-receptor expression. RNA microarray screening adjustments and alterations in in chemokine co-receptor so as to screen for general TNF induced TNF was added to the cell culture medium expression. RNA microarray screening revealed revealed that changes in syndecan expression occurred, but that glypican expression remained adjustments and changes expression occurred, but that glypican RNA microarray screening that changes in syndecan in chemokine GAG co-receptor expression.expression remained Topoisomerase Storage & Stability unchanged unchanged that Supplemental Material to get a total list butall changes). As an essential internal revealed (see comprehensive syndecan expression occurred, of that glypican expression remained (see Table S1 for any modifications in list of all modifications). As a crucial internal control, the expression of manage, the expression of CXCL8 was found to become 33-fold up-regulated following TNF stimulation unchanged (see Supplemental up-regulated following TNF stimulation important corresponds CXCL8 was identified to be 33-fold Material for a complete list of all adjustments). As an [51]. Thisinternal (data not shown). This corresponds to identified to be 33-fold up-regulated following TNF stimulation manage, the expression of CXCL8 was earlier findings, see as an example Reference [51]). RT-qPCR to preceding findings, see for examplePARP2 Synonyms quantitate changes in SDC gene expression. For was applied to Reference [52]. RT-qPCR making use of SDC primers this implies, employing SDC shown). This corresponds to earlier findings, see for example Reference [51]). RT-qPCR (data not primers was applied to quantitate microvascular wasgene expression. Forchanges in SDC gene expression. For this ng/mL for adjustments in SDC applied cells (HMVECs) have been again stimulated with TNF endothelial cells human SDC primers endothelial to quantitate this implies, human microvascular 50 suggests, applying (HMVECs) have been once more stimulated with TNF 50 ng/mL for stimulated with induce an inflammatory four hours to induce endothelial cells (HMVECs) were again four to enable TNF 50 ng/mLof HS human microvascular an inflammatory response in vitro and hours to investigation for response in vitroto induceenable investigation of HSin vitroTNF to expression under inflammatory four hours and to below inflammatory conditions. and treatment resulted in HS proteoglycan expression an inflammatory response proteoglycan enable investigation aof2.7-fold proteoglycan expression under whilst SDC2 conditions. was therapy resulted in a when situations. in SDC4 therapy resulted in a two.7-fold improve in SDC4 transcription, 2.7-fold 1). boost TNF transcription, inflammatory expression TNFdecreased five.8-fold (see Figure SDC2 improve in SDC4 in accordance with SDC2 expression was decreased 5.8-fold (see Figure the These findings were transcription, while the gene These findings had been in accordance Material). expression was decreased five.8-fold (see Figure 1). array measurements (see Supplementalw.