Icles. We’ve got not too long ago enhanced the contrast and spatial resolution of SPIRI by pupil function engineering and computational imaging. Procedures: In SPIRI, the interference of light reflected from the sensor surface is modified by the presence of particles generating a distinct signal that reveals the size in the particle that is definitely not otherwise visible beneath a traditional microscope. Working with this instrument platform, we have demonstrated label-free identification and visualization of a variety of viruses in multiplexed format in complex samples in a disposable cartridge. Not too long ago, our technologies was applied to detection of exosomes and commercialized by Nanoview Biosciences for quantified measurement of exosomes on dry sensor chips. We are at present focusing onISEV2019 ABSTRACT BOOKvarious in-liquid detection at the same time as further improvement from the approach applying pupil function engineering. Final results: By acquiring several photos having a partitioned pupil (resulting in structured illumination) and computational imaging, we’ve demonstrated important improvement in visibility of low-index nanoparticles in liquid. In addition, spatial resolution has been enhanced beyond the diffraction limit approaching one hundred nm inside the visible microscopy. We have created compact and cheap sensor chips and microfluidic cartridges enabling for study of biological particles (exosomes and other extracellular vesicles) directly within the bodily fluids without the need of labels. Summary/Conclusion: In summary, we’ve demonstrated improved visibility of exosomes in SPIRI utilizing pupil function engineering. Funding: EU-INDEXuse of several recognition events in mixture with signal amplification allows detection of exosomes with high specificity and sensitivity. Summary/Conclusion: Here, we talk about the δ Opioid Receptor/DOR Biological Activity application of proximity assays for sensitive detection of exosomes in MNK1 list physique fluids, to visualize the uptake of exosomes by cells, along with the potential of such method to become employed to far better recognize the biology in the exosomes and to identify exosomes as disease biomarkers.OF22.A 96 well plate format lipid quantification assay with improved sensitivity for standardization of experiments with extracellular vesicles Tamas Visnovitza, Xabier Osteikoetxeab, Barbara W. S arc, Judith Mihalyd, P er Lrincze, Krisztina V. Vukmana, Eszter nes T ha, Anna Koncza, Inna Sz sf, Robert Horv hf, Zoltan Vargag and Edit I Buz c Semmelweis University, Dept. of Genetics, Cell- and Immunobiology, Budapest, Hungary; bAstraZeneca, Macclesfield, UK; cSemmelweis University, Budapest, Hungary; dRCNS HAS, Budapest, Hungary; e Department of Anatomy, Cell and Developmental Biology, E v Lor d University, Budapest, Hungary; fNanobiosensorics Laboratory MTA-EKMFA, Budapest, Hungary; gResearch Centre for Organic Sciences, Hungarian Academy of Sciences, Budapest, HungaryaOF22.Proximity assays for detection and characterization of exosomes Masood Kamali-Moghaddam, Ehsan Manouchehri, Alireza Azimi, Qiujin Shen, Radiosa Gallini and Claudia Fredolini Uppsala University, Uppsala, SwedenIntroduction: Exosomes acquire an enhanced focus in standard biology at the same time as in medicine. They are shown to be involved in numerous biological processes, and are proven to hold wonderful potentials as diagnostic and therapeutic tools. Having said that, there is certainly an unmet want for new and improved technologies for quantitative and qualitative characterization of exosomes to meet challenges related to these vesicles, for example low concentrations in body f.