H an age selection of 55.82 9.31. The NCR group integrated 213 CAD individuals subjected to PCI that responded to clopidogrel as an inhibitor of platelet aggregation. The NCR group individuals have been 54 females and 159 males with an average age of 57.67 7.99. 2.three. Blood analysis Within the morning and ahead of subjecting for PCI procedure, a sample of five milliliters of venous blood was withdrawn from each and every patient. The blood sample was divided into 3 parts, two ml placed in an EDTA tube for DNA extraction, 1ml in a lithium-heparin tube for platelet aggregation test using Multiplateanalyzer from Roche firm, and the final two ml were placed inside a straight tube for serum VEGFR2 and other parameters [17]. The ELISA method applied to detect serum VEGFR2 via RayBiohuman VEGFR2 ELISA kit. Moreover, estimation of body mass index (BMI) by way of equation formula for BMI which can be weight in kilograms divided by height in meters squared while serum lipid profile levels which includes CDK16 Formulation low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglyceride (TG), cholesterol, and very-low-density lipoprotein (VLDL) were analyzed automatically inside the hospital for all the participants in this study making use of BIOLABO kits following manufacturer directions. Regarding platelet aggregation test, it was performed applying MULTIPLATEanalyzer by Roche company utilizing the ADP particular test with its reagents. two.four. Genotype determination DNA extraction was performed ErbB2/HER2 Compound employing a precise kit for DNA purification (Promega, USA). The protocol described by the manufacturer was followed. The determination of the KDR rs1870377 genetic variant was carried out using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) approach. The amplification of a DNA sequence containing the KDR rs1870377 genetic variant was performed applying precise primers (Promega, USA). The sequence of forwarding primer is ‘5TGCAAGTCCTCCACACTTCTCCAT-3,’ plus the reverse primer is ‘5-AAGGAGGCCAGTGGCTTCTAAGTT-30 , and also the PCR consisted 35 cycles of denaturation at 95 C for 1 min, primer annealing at 63 C for 1 min and lately extension at 72 C for 1 min, as described previously [18]. In accordance with the manufacturer protocol, the PCR merchandise were digested by certain restriction enzyme AluI (Promega, USA). The restricted PCR items have been electrophoresis by way of three agarose gel. The genotyping final results were confirmed via DNA Sanger sequencing by Applied Biosystems Model (ABI3730x1) (Macrogen, South Korea). 2.5. Statistical evaluation Continuous variables have been illustrated by mean SD. Student’s t-test to express the signifies variance amongst NCR and CR. ANOVA test was applied for describing level rates of continuous parameters in genotypes By way of the SPSS v. 25.0 computer software (Chicago, IL SPSS Inc). Genotype distribution and allele frequency expression performed by non-numerical variables. chi-squared test to assess the existence of differences of these variables. If p worth was 0.05, then variations are considered substantial. two.5.1. Logistic regression (Multinomial) Logical regression was obtained by SPSS application, to predict the relevance of allele frequencies and genotype to CR with numerous models of inheritance. The rs1870377 SNP of KDR gene. Odds ratio (OR) would be the expression for the results relating to dissection for allele frequencies and genotype allocation, P-value and confidenceW. Al Awaida et al.Heliyon 7 (2021) einterval (CI – 95 ). Outcome adjustment for sex, age, BMI, HT, DM and smoking, OR, CI 95 and P-.