Y junction DNA binding”, and “DNA clamp loader activity”. In addition, “carbohydrate transmembrane transport”, “carbohydrate: proton symporter activity”, and “sugar transmembrane transporter activity” were also substantially enriched. These benefits showed that the infection of SsHADV-1 may activate the DNA harm response and enhance the carbohydrate acquisition of strain DT-8.Figure two. The GO Dopamine β-hydroxylase custom synthesis CB2 review enrichment evaluation of DEGs. (a) The GO enrichment analysis from the up-regulated genes. (b) The GO enrichment analysis of the down-regulated genes.J. Fungi 2021, 7,8 ofThe 1369 down-regulated genes had been drastically enriched to 10 finish node GO terms (Figure 2b, Table S3) and 3 substantially enriched GO terms have been related to the structure and function with the ribosome, namely, “ribosomal subunit,” “cytosolic ribosome,” and “structural constituent of ribosome.” Meanwhile, “translation” was also the enriched GO term. There have been also two GO terms linked with carbohydrate metabolism, namely, “carbohydrate binding” and “carbohydrate metabolic process.” These GO terms might be associated for the reduced development of strain DT-8. three.three. KEGG Enrichment Evaluation of DEGs The KEGG enrichment analysis in the up-regulated S. sclerotiorum genes showed comparable outcomes towards the GO enrichment analysis. For the 1741 up-regulated genes, there were 12 considerably enriched pathways, of which eight pathways were related to DNA replication and DNA repair, such as “replication and repair”, “DNA repair and recombination proteins”, “DNA replication”, “mismatch repair”, “nucleotide excision repair”, “homologous recombination”, “DNA replication proteins” and “nucleotide metabolism” (Figure 3a, Table S4). These pathways also showed that the infection of SsHADV-1 may well activate the DNA harm response of strain DT-8.Figure 3. The KEGG enrichment evaluation of DEGs. (a) The KEGG enrichment analysis of your up-regulated genes. (b) The KEGG enrichment evaluation from the down-regulated genes.For the down-regulated genes, comparable for the GO enrichment analysis, the “carbohydrate metabolism”, “starch and sucrose metabolism”, “ribosome”, “translation”, and “translation factors” had been the enriched pathways. In addition, the “lipid biosynthesis proteins” have been also enriched (Figure 3b, Table S5). These pathways also may be associated to the reduced development of strain DT-8. three.four. The Important Non-Homologous End Joining (NHEJ) Genes Had been Up-Regulated in Strain DT-8 Ku70-Ku80 is really a DNA-binding heterodimer that forms a complicated using the DNA repair protein XRCC4 as well as the DNA ligase 4 to activate the NHEJ pathway for the repair of DNA double-strand breaks [64]. Inside the S. sclerotiorum genome, you will discover two Ku70/Ku80 homologs (ssKu70, SS1G_02717; ssKu80, SS1G_07128) [65], one DNA repair protein XRCC4 (SS1G_02074), and a single DNA ligase 4 (SS1G_03342) (Table S6). When compared with these in strain DT-8VF, all the important NHEJ genes had been up-regulated in strain DT-8 (Figure 4). This outcome recommended that the infection of SsHADV-1 activated the NHEJ pathway in strain DT-8.J. Fungi 2021, 7,9 ofFigure 4. The expression profiles from the essential NHEJ genes.three.five. Many of the Antiviral RNA Silencing Genes Were Down-Regulated in Strain DT-8 RNA silencing is identified as an adaptive defense mechanism against foreign nucleic acids, including viruses in animals, fungi, and plants [66,67]. Inside the S. sclerotiorum genome, there had been two Dicer-like (Dcl) genes, two argonaute-like (Agl) genes, and 3 RNAdependent RNA polymerase (RDR) genes [46]. When compared with.