Recommended that the HpaBC complex has in vivo activity towards p-CA, and Af, with higher catalytic efficiency. Our Our benefits showed activity towards p-CA, N, N, and Af, with high catalytic efficiency. results showed that that these substrates (except anthocyanin) may very well be converted towards the PARP2 drug corresponding orthothose substrates (except anthocyanin) may be converted to the corresponding ortho-prodproducts the optimaloptimal circumstances. Similarly, the conversion of those by P2 3ucts under below the conditions. Similarly, the conversion of those substrates substrates by P2 3-carrying also drastically higher than larger than that of P2-carrying strains. carrying strains was strains was also drastically that of P2-carrying strains. The converThe conversion to E was 58 , followed by that for Af by that three.14), p-CA (32.93 2.01), sion rate from N rate from N to E was 58 , followed (35.2 for Af (35.2 3.14), p-CA (32.93 2.01), DHK (23.74 1.75) and K (23.84 0.88), even though substrate PEL was not PEL DHK (23.74 1.75) and K (23.84 0.88), though activity around the activity on the substratedewas not detected (Table 2). These final results provided proof that the HpaBC complex had tected (Table two). These results provided proof that the HpaBC complicated had in vivo in vivo activity towards monohydroxylated flavanol and flavonol. activity towards monohydroxylated flavanol and flavonol.Molecules 2021, 26, 2919 Molecules 2021, 26, x FOR PEER REVIEW9 of 13 9 ofFigure six. HPLC analysis of the enzymatic products of the HpaBC complex, when feeding with distinctive substrates. HPLC Figure six. HPLC evaluation on the enzymatic products with the HpaBC complex, when feeding with different substrates. HPLC chromatogram chromatogram (left) and standard compound (right) analyses on the enzymatic reaction. N, E,E, K, Q, DHK, DHQ, and Af standard compound (appropriate) analyses in the enzymatic reaction. N, K, Q, DHK, DHQ, C C and were monitored at at nm, and p-CA and CA were monitored at 340 340 The substrates and and corresponding items Af were monitored280280 nm, and p-CA and CA were monitored at nm. nm. The substrates corresponding goods had been were detected by HPLCLC-MS. The ortho-hydroxylation activities of (a): p-CAp-CA to (b): N to N to E; (c): Af to C;K to Q; detected by HPLC and and 2021, 26, x FOR PEER Evaluation Molecules 2021, 26,Molecules LC-MS. The ortho-hydroxylation activities of (a): to CA; CA; (b): E; (c): Af to C; (d): (d): K x FOR PEER Critique to Q;(e): DHKDHK to DHQ. Final substrate concentration of 80 mg-1, three. = 3. L and and (e): to DHQ. Final substrate concentration of 80 mg -1 , n = n10 ofTable two. The yield and conversion price of ortho-hydroxylated flavonoids for the HpaBC complex Table two. The yield and2. The yield and conversion rate of ortho-hydroxylated flavonoidscomplex when feeding with difTable conversion price of ortho-hydroxylated flavonoids for the HpaBC for the HpaBC complicated when feedin when feeding with distinct substrates. The horizontal lines in the table PKCĪ· web indicate that no activities has ferent substrates. The horizontal lines horizontal lines in the table indicate that no activities has Data are shownData are sho ferent substrates. The within the table indicate that no activities has been detected. been detected. as the been signifies s.d.s (n indicates s.d.s (n =Data are shown because the suggests s.d.s (n = three). = three). detected. 3).SubstratesSubstrates SubstratesProducts ProductsYield Yield Conversion Rate ( ) Conver L Conversion Merchandise (mg )(mg-1) Yield (mg-1) Price ( ) L-2.