Tegrative modeling can contribute a good deal to solving this challenge. We propose that members in the smFRET neighborhood who’re serious about applying smFRET for integrative structural biology participate in the CASP competitors. Involvement could progress in numerous stages: (1) Predicting single- and multi-state structural models: the smFRET neighborhood will only submit distances that may be evaluated with respect to the identified (but undisclosed) crystal structure(s). (two) Predicting ensembles as in the case of CBM56: for targets that happen to be identified as complicated by the predictors and for which numerous possible folds are submitted devoid of a clear winner, a FRET-assisted round could be insightful exactly where the FRET distances distributions might be used as an experimental `ground truth’ for checking whether or not several conformations in an exchange are present. These recommendations apply mainly to CDK14 site present and future practitioners of smFRET-driven integrative modeling. That being stated, smFRET is among numerous biophysical approaches that can deliver experimental restraints in integrative modeling (XL-MS, single-particle cryoEM, NMR, SAXS). Consequently, we propose that, at a later stage, an all-biophysics integrative structural biology competitors be established.SmFRET meetingsSeveral gatherings of FRET practitioners at the Annual Biophysical Society Meetings, supported by the Biological Fluorescence subgroup, provided a platform for preparing future activities and establishing the FRET community. As further joint actions, satellite meetings towards the Conference on Solutions and Applications in Fluorescence (MAF) have been organized to discuss practices, standards, competitions, and joint publications. We envision an occasional devoted meeting for the smFRET neighborhood, which include the Bunsen meetings on FRET held in 2011 as well as the international discussion meeting in 2016 at the Max Planck Institute for Biophysical Chemistry in Gottingen, Germany (http:// fret.uni-duesseldorf.de/cms/home.html). However, to open these meetings to smFRET practitioners outside of Europe, we propose to rotate the venue amongst continents. We also recommend employing the satellite meetings and workshops to disseminate information (details of accurate FRET measurements, common practices, standards and competitions) and to offer newcomers the possibility to interact with the skilled researchers inside the field.Lerner, Barth, Hendrix, et al. eLife 2021;ten:e60416. DOI: https://doi.org/10.7554/eLife.35 CDK16 MedChemExpress ofReview ArticleBiochemistry and Chemical Biology Structural Biology and Molecular BiophysicsInspired by the on-line seminars emerging in response to the COVID-19 pandemic, smFRET webinars and web conferences open to all need to be pursued. They supply FRET researchers the one of a kind chance to attend and socialize practically and would be a forum for superior scientific practice of open science for the FRET community.Special challenges in journalsTo further stimulate newcomers to engage in advanced smFRET experiments, the FRET community could benefit by hosting specific challenges in journals devoted to information analyses (e.g., Information in Brief, Procedures in Molecular Biology, or Nature Protocols). Here, several laboratories can describe typical datasets or protocols for the strategies they have developed. Also, journals disseminating methodologies and protocols from A-to-Z by means of video recordings might be helpful. By way of example, there is a particular situation focusing on FRET planned within the Journal of Visualized Experiments (https://www.jove.com/ solutions.