Metabolism; pu, purine metabolism; a, amino acid metabolism; gi, genetic facts
Metabolism; pu, purine metabolism; a, amino acid metabolism; gi, genetic information and facts processing; t, translation; l, lipid metabolism; tca, citrate cycle; e, power metabolism; c, carbon fixation, within this case reductive glyoxylate cycle.The response to a combination of Zn and PO4 3- scarcity was considerably diverse, particularly for proteins in high abundance below PO4 3- scarcity (Figure 5A; Table 2). None on the proteins were exactly the same because the Tetu et al. (2009) transcriptomestudy. Most notable was the influence on two crucial PO4 3- acquisition proteins: the alkaline phosphatase and phosphate 4-1BB Accession transporter described above (Figures 7B,C), which had been only modestly affected by PO4 3- limitation beneath Zn scarcity. InFrontiers in Microbiology | Microbiological ChemistryDecember 2013 | Volume four | Article 387 |Cox and SaitoPhosphatezinccadmium proteomic responsesA SYNWFold adjust in protein relative abundance (low PO43-high PO43- )bacterial metallothioneinSYNW2391 alkaline phosphataseSYNW0799 G3P dehydrogenase SYNW0953 SwmB SYNW0085 SwmA SYNW0156 phosphorylase SYNW2224 porin SYNW0160, SYNW1119 SYNW1213, SYNW1815, SYNW0406, SYNW2508 SYNW1018 PstSB SYNWlog2 fold change in transcript abundance (P-stressedP-replete) proteintranscript much more than two-fold in both 1:1 equal fold abundance proteintranscript a lot more than two-fold in transcriptRelative Protein Abundance14 12 10 eight 6 4 2putative alkaline phosphataseFIGURE 6 | Fold modify in protein relative abundance (this experiment) as ratio of low phosphate to high phosphate vs. log2 fold transform in gene relative abundance (Tetu et al., 2009) as ratio of P-stressed to P-replete. Pink dots represent proteinstranscripts much more than two-fold abundant in each protein and transcript data. Black dots represent proteinstranscripts a lot more than two-fold abundant in transcript data. Red dashed line indicates a 1:1 equal fold abundance. SYNW0160 conserved hypothetical protein; SYNW1119 6-phosphogluconate dehydrogenase; SYNW1213 thioredoxin peroxidase; cIAP-2 Compound SYNW1815 ABC transporter, substrate binding protein, phosphate; SYNW0406 hypothetical protein; SYNW2508 molecular chaperone DnaK2, heat shock protein hsp 70-2. See Tables 1, 2.C SYNW1018 ABC transporter,one hundred 80 60 40 20substrate binding protein, phosphate (PstS)addition, bacterial metallothionein didn’t raise in abundance with scarce PO4 3- (Figure 7A). Collectively these responses recommend a regulatory response to Zn that prevents synthesis on the metalloenzyme alkaline phosphatase when a needed metal cofactor is absent. We need to caveat that the metal atom center has not been demonstrated to be Zn for this alkaline phosphatase isoform, as well as other metals could also have functionality (and even be the “intended” metal), and that marine cyanobacteria like Synechococcus sp. WH8102, S. bacillaris, and Prochlorococcus MED4 have all been shown to possess tiny to no Zn requirement (Sunda and Huntsman, 1995; Saito et al., 2002, 2003), even though this has not been tested below circumstances of organic PO4 3- utilization. In addition, our final results recommend that the hypothetical protein SYNW1661 may be involved inside the phosphate pressure response in the presence of zinc (Table 1). Together, these observations recommend that Zn nutritional levels are connected for the PO4 3- response within this cyanobacterium. Several proteins decreased in abundance in response to PO4 3- scarcity beneath low Zn circumstances, including many ribosomal proteins identified in decrease abundance which can be most likely related towards the depress.