Ncer cell lines in vitroGIOVANNI VANNI FRAJESE1, MONICA BENVENUTO2, MASSIMO FANTINI2, ELENA AMBROSIN1, PAMELA SACCHETTI3, LAURA MASUELLI3, MARIA GABRIELLA GIGANTI2, ANDREA MODESTI2 and ROBERTO BEIDepartment of Sports Science, Human and Health, University of Rome `Foro Italico’, Rome I-00135; Division of Clinical Sciences and Translational Medicine, University of Rome `Tor Vergata’, Rome I-00133; three Division of Experimental Medicine, University of Rome `Sapienza’, Rome I-00185, Italy Received April 17, 2015; Accepted February eight, 2016 DOI: 10.3892/ol.2016.Abstract. Ascorbic acid (A) has been demonstrated to exhibit anti-cancer activity in association with chemotherapeutic agents. Potassium (K) is really a regulator of cellular proliferation. In the present study, the biological effects of A and K bicarbonate, alone or in combination (A+K), on breast cancer cell lines were evaluated. The survival of cancer cells was determined by sulforhodamine B cell proliferation assay, while evaluation on the cell cycle distribution was conducted by means of fluorescence-activated cell sorting. In addition, the expression of signaling proteins was analyzed upon treatment. The outcomes indicated that there was a heterogeneous response of the distinct cell lines to A and K, plus the greatest effects were accomplished by A+K and a remedy. The interaction in between A+K indicated an additive or synergistic effect. Also, A+K improved the percentage of cells in the sub-G1 phase from the cell cycle, and was one of the most helpful therapy in activating the degradation of poly(adenosine diphosphate-ribose) polymerase-1. In the breast cancer cell line MCF-7, A+K induced the look on the 18 kDa isoform of B-cell lymphoma-2-associated X protein (Bax), which is a far more potent inducer of apoptosis than the full-length Bax-p21. The effects of A and K around the phosphorylation of extracellular signal-regulated kinase (ERK)1 and ERK2 were heterogeneous. In addition, treatment with K, A and A+K inhibited the expression of nuclear factor- B. Overall, the results of the present study indicated that K potentiated the anti-tumoral effects of A in breast cancer cells in vitro.Introduction The usage of ascorbic acid (A) as an anti-cancer agent has been analyzed in the final 50 years (1,two). Previous epidemiological research have demonstrated that dietary administration of A exerts a preventive impact on a number of tumors (1,2). Even so, Cameron and Pauling (3) have argued its role as a therapeutic anti-cancer agent, in agreement with preceding research disproving its possible role as an anti-cancer agent (4,five).Protein A Magnetic Beads custom synthesis These research were performed supplementing the eating plan with higher doses of orally administered vitamin C, which allows to attain saturation at plasma concentrations of 1 g/day, though greater doses of vitamin C are excreted (six,7).ER beta/ESR2 Protein manufacturer Blood concentrations of vitamin C at mM levels are only probable to obtain by means of intravenous injections of high doses of your compound (8,9).PMID:23329319 In current years, the interest on A and its effects on cancer growth has improved (10). A has been lately demonstrated to become very helpful in cancer therapy in association with frequently made use of chemotherapeutic agents in ovarian tumors (11). Similarly to the case of A, the part of potassium (K) in cancer has remained unclear considering the fact that Cone (12) reported in 1971 higher levels of Na+ and lower levels of K+, Ca 2+ and Zn in cancer cells, compared with wholesome cells. K+ is capable of acting as an anti-apoptotic and as a pro-apoptotic agent (13,1.