Receptor-; TMA: tissue microarray; IHC: immunohistochemistry; OR: odds ratio; HR: hazard ratio; CEA: carcinoembryonic antigen; VGSCs: voltage-gated sodium channels; E2: -estradiol; DAB: three,3-diaminobenzidine tetrahydrochloride; CI: confidence interval; DFS: illness free of charge survival; OS: general survival. Authors’ contributions JP and YF drafted the manuscript; XW, RZ and WJ participated in clinical information and paraffin-embedded specimens collection; JP, QO and YF played a important role in tissue microarray construction and immunohistochemistry examination; JP, YF and QZ carried out the information evaluation; YF and ZL supervised the research plan and edited the manuscript; DW, ZP and YF had substantial roles within the study design and style and manuscript overview. All authors read and approved the final manuscript. Author facts 1 Department of Colorectal Surgery, State Essential Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yatsen University Cancer Center, 651 Dongfeng Road East, Guangzhou 510060, Guangdong, P. R. China. two Department of Experimental Investigation, Sun Yat-sen University Cancer Center, Guangzhou 510060, Guangdong, P. R. China. three Department of Statistics, School of Public Overall health, Guangzhou Medical University, Guangzhou 510182, Guangdong, P. R. China. Acknowledgements We deeply appreciate the help from all colleagues of the Division of Colorectal Surgery at Sun Yat-sen University Cancer Center, who have worked in getting the tissue specimens and supplied it towards the existing study. The authors would prefer to thank the two pathologists, Shixun Lu and Peng Li from Department of Pathology at Sun Yat-sen University Cancer Center for the TMA-IHC evaluation. Competing interests The authors declare that they’ve no competing interests. The authenticity of this short article has been validated by uploading the important raw information onto the Investigation Information Deposit public platform (http://www.researchdata.org.cn), using the Approval Number as RDDB2017000048. Availability of information and materials The datasets analysed through the present study were readily available in the corresponding author on affordable request. Any individual who is keen on the data must make contact with [email protected] and [email protected]. Consent for publication Not applicable.Ethics approval and consent to participate This study was undertaken in accordance using the ethical requirements in the Globe Medical Association Declaration of Helsinki.BMP-2 Protein MedChemExpress Informed consents ahead of initial therapy for using tissue samples had been requested plus the study approval was obtained in the Institutional Analysis Ethics Committee of Sun Yat-sen University Cancer Center (Approval Quantity: GZR-2016-071).MFAP4 Protein manufacturer Funding This perform was supported by a grant from the Sun Yat-sen University Clinical Investigation 5010 Plan (Grant No.PMID:24268253 2015024), a grant of Guangzhou Science and Technologies Program Projects (Overall health Healthcare Collaborative Innovation Plan of Guangzhou) (Grant No. 201400000001-4) as well as a grant of National Organic Science Foundation of China (Grant No. 81772595). Received: 15 February 2017 Accepted: 21 OctoberReferences 1. Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, et al. Cancer statistics in China, 2015. CA Cancer J Clin. 2016;66(2):1152. two. Xu J, Qin X. Specialist consensus on robotic surgery for colorectal cancer (2015 edition). Chin J Cancer. 2016;35:23. three. Xu RH, Shen L, Li J, Xu JM, Bi F, Ba Y, et al. Professional consensus on maintenance treatment for metastatic colorectal cancer in China. Chin J.