Species: Human Expressing host: HEK293 cells Description: Recombinant Human Carbonic Anhydrase 9 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln38-Asp414) of human Carbonic Anhydrase IX/CA9 (Accession #NP_001207.2) fused with a 6HisAvi tag at the C-terminus. Tag: C-His&Avi Purity: > 95% by SDS-PAGE. Endotoxin: Please contact us for more information. Biological Activity: Measured by its esterase activity. The specific activity is >84 pmoles/min/g, as measured with 1 mM 4-Nitrophenyl acetate and 1 g enzyme at 400 nm in 100 L of 12.5 mM Tris, 75 mM NaCl, pH 7.5. preparation: Lyophilized from a 0.22 m filtered solution of PBS, pH 7.4. Storage Store the lyophilized protein at -20C to -80C for long term.
After reconstitution, the protein solution is stable at -20C for 3 months, at 2-8C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : Carbonic Anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the Carbonic Anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers. GeneID 768 Swiss Prot Q16790 Alias: CA9;CAIX;MN; MNMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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