Species: Human Expressing host: HEK293 cells Description: Recombinant Human Guanylyl cyclase C/GUCY2C Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser24-Gln430) of human GUCY2C/Guanylyl cyclase (Accession #NP_004954.2) fused with a 6HisAvi tag at the C-terminus. Tag: C-His&Avi Purity: > 95% by SDS-PAGE. Endotoxin: Please contact us for more information. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human GUCY2C at 1 g/mL (100 L/well) can bind GUCY2C Rabbit pAb with a linear range of 1-144 ng/mL. preparation: Lyophilized from a 0.22 m filtered solution of PBS, pH 7.4. Storage Store the lyophilized protein at -20C to -80C for long term.
After reconstitution, the protein solution is stable at -20C for 3 months, at 2-8C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer. GUCY2C may represent a new target for anti-obesity pharmacotherapy. GeneID 2984 Swiss Prot P25092-1 Alias: GUCY2C;DIAR6;GC-C;GUC2C;MECIL;MUCIL;STARMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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