Species: Human Expressing host: HEK293 cells Description: Biotinylated Recombinant Human CD4 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Lys26-Trp390) of Human CD4 fused with His and Avi tag at the C-terminal. Tag: C-His&Avi Purity: > 95% by Tris-Bis PAGE;> 95% by SEC-HPLC Endotoxin: Biological Activity: Immobilized Biotinylated Human CD4 at 0.5 g (100 L/Well) on the plate.Dose response curve for Anti-CD4 Ab, with the EC50of 21.0 ng/mL determined by ELISA. preparation: Lyophilized from a 0.22 m filtered solution of PBS, pH 7.4. Storage Store the lyophilized protein at -20C to -80C for long term.
After reconstitution, the protein solution is stable at -20C for 3 months, at 2-8C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : GeneID 920 Swiss Prot P01730 Alias: CD4;CD4mutMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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