Species: Human Expressing host: HEK293 cells Description: Recombinant Human FGFR-2/KGFR/CD332 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg22-Glu377) of human FGFR-2 (Accession #NP_000132.3) fused with a Fc, 6His tag at the C-terminus. Tag: C-hFc&His Purity: > 97% by SDS-PAGE. Endotoxin: Biological Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human FGF1 at 5 g/mL (100 L/well) can bind Recombinant Human FGFR2 with a linear range of 0.8-2.5 g/mL.
2.Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED50for this effect is typically 0.256-0.991 ng/mL. preparation: Lyophilized from a 0.22 m filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : The protein is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. GeneID 2263 Swiss Prot P21802-1 Alias: BBDS;BEK;BFR-1;CD332;CEK3;CFD1;ECT1;JWS;K-SAM;KGFR;TK14;TK25;FGFR2MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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