Species: Human Expressing host: HEK293 cells Description: Active Recombinant Human Latent TGF-beta 1(C33S) Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Leu30-Ser390(C33S)) of human TGF-beta 1 (Accession #NP_000651.3) fused with an 8His tag at the N-terminus. Tag: N-His Purity: > 95% by SDS-PAGE. Endotoxin: Biological Activity: 1.Measured by its ability to inhibit cell proliferation of TF-1 human erythroleukemic cells. The ED50for this effect is typically 0.5-2 ng/mL, corresponding to a specific activity of 5.0105~2.0106units/mg.
2.Measured by its binding ability in a functional ELISA. Immobilized recombinant human TGFB1 at 2 g/mL (100 L/well) can bind recombinant human TGFBR2 with a linear range of 1-5 ng/mL.
3.Measured by its ability to inhibit the IL-4(Catalog: RP01161)-dependent proliferation of HT-2 mouse T cells. The ED50for this effect is 9.75-39 ng/mL, corresponding to a specific activity of 2.6104~1.0105units/mg. preparation: Lyophilized from a 0.22 m filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile 4 mM HCl. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : GeneID 7040 Swiss Prot P01137 Alias: TGFB1; CED; DPD1; LAP; TGFB; TGFbeta; transforming growth factor beta-1;TGF-beta 1;CED;DPD1;LAP;TGFB;TGFbeta;TGF-MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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