Species: Human Expressing host: HEK293 cells Description: Recombinant Human Neuropilin-1/NRP1/VEGF165R/CD304 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Phe22-Lys644) of human Neuropilin-1/NRP1 (Accession #NP_001019799) fused with a 6His tag at the C-terminus. Tag: C-His Purity: > 97% by SDS-PAGE. Endotoxin: Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human NRP1 Protein at 2 g/mL (100 L/well) can bind NRP1 Rabbit pAb with a linear range of 1.9-98.96 ng/mL.
2.Measured by its binding ability in a functional ELISA.Immobilized human VEGF165 at 1g/mL (25 L/well) can bind Human CD304 with a linear range of 0.46-15.8ng/mL. preparation: Lyophilized from a 0.22 m filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. GeneID 8829 Swiss Prot O14786 Alias: BDCA4;CD304;NP1;NRP;VEGF165R;Neuropilin-1;NRP1MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
IL-21R Protein
Hepcidin/HAMP Protein
Popular categories:
Neurokinin B
IFN-lambda 2/IL-28A