Species: Mouse Expressing host: E. coli Description: Active Recombinant Mouse IL-1 beta Protein is produced byE. coliexpression system. The target protein is expressed with sequence (Val118-Ser269) of mouse IL-1 beta (Accession #NP_032387.1) fused with 6His tag at the C-terminus. Tag: C-His Purity: > 95% by SDS-PAGE. Endotoxin: Biological Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Mouse IL1B at 10 g/mL (100 L/well) can bind Human IL1R2 with a linear range of 0.03125-0.8036 g/mL.
2.Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50for this effect is 42.1-168.4 pg/mL, corresponding to a specific activity of 5.94106-2.37107units/mg. preparation: Lyophilized from a 0.22 m filtered solution of PBS, pH 7.4. Storage Store the lyophilized protein at -20C to -80C for long term.
After reconstitution, the protein solution is stable at -20C for 3 months, at 2-8C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family.This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. GeneID 16176 Swiss Prot P10749 Alias: IL-1 beta;IL-; Il-1b; IL-1beta;Il1bMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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