Species: Human Expressing host: HEK293 cells Description: Recombinant Human Cyr61/CCN1 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Thr25-Asp381) of human Cyr61/CCN1 (Accession #O00622) fused with an Fc tag at the C-terminus. Tag: C-Fc Purity: > 95% by SDS-PAGE. Endotoxin: Biological Activity: Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Recombinant Human Cyr61/CCN1 Fc Chimera immobilized at 1.5 g/mL (100 L/well), will mediate >25% BALB/3T3 cell adhesion (3 x 104cell/well added). preparation: Lyophilized from a 0.2 m filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. GeneID 3491 Swiss Prot O00622 Alias: CYR61; CCN1; GIG1; IGFBP10; protein CYR61;CCN1;GIG1;IGFBP10MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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