Species: Human Expressing host: E. coli Description: Recombinant Human Visfatin/Nampt Protein is produced byE. coliexpression system. The target protein is expressed with sequence (Asn2-His491) of human Visfatin/Nampt (Accession #P43490) fused with no additional amino acid. Tag: NO-tag Purity: > 95% by SDS-PAGE. Endotoxin: Biological Activity: Measured in a cell proliferation assay using RPMI 8226 cells. The ED50 for this effect is 1.726.89 ng/mL,corresponding to a specific activity of 1.45105~5.81105units/mg. preparation: Lyophilized from a 0.2 m filtered solution of 20mM HEPES, 150mM NaCl, pH8.0.Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : Pre-B cell colony enhancing factor (PBEF) was originally identified as a cytokine that potentiated the clonalexpansion and differentiation of pre-B cells, but it is also acknowledged to be the ubiquitous intracellularenzyme nicotinamide phosphoribosyltranferase (NAMPT) and the adipokine visfatin . PBEF is constitutivelyexpressed in the fetal membranes where its greatest expression is in the amnion. It has intracellular andextracellular forms. Most of the intracellular functions of PBEF are due to its role as a Nampt which can induceangiogenesis through upregulation of VEGF and VEGFR and secretion of MCP-1. Extracellular PBEF has beenshown to increase inflammatory cytokines, such as TNF- , IL-1 , IL-16, and TGF- 1. PBEF also increases theproduction of IL-6, TNF- , and IL-1 in CD14+ monocyctes, macrophages, and dendritic cells, enhances theeffectiveness of T cells. GeneID 10135 Swiss Prot P43490 Alias: NAMPT;1110035O14Rik;PBEF;PBEF1;VFMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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