Species: Human Expressing host: E. coli Description: Recombinant Human Amphiregulin/AREG/AREGB/CRDGF/SDGF/AR Protein is produced byE. coliexpression system. The target protein is expressed with sequence (Ser101-Lys198) of human Amphiregulin/AREG/AREGB/CRDGF/SDGF/AR (Accession #P15514) fused with an initial Met at the N-terminus. Tag: No tag Purity: > 95% by SDS-PAGE. Endotoxin: Biological Activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50for this effect is 5-15 ng/mL. preparation: Lyophilized from a 0.2 m filtered solution of 20mM PB,150mM NaCl,pH7.4.Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : GeneID 374 Swiss Prot P15514 Alias: AREG;AR;AREGB;CRDGF;SDGF;amphiregMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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