Species: Human Expressing host: HEK293 Description: Recombinant human CCL2/MCP-1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln24-Thr99) of human CCL2/MCP-1 (Accession #) fused with additional amino acid free. Tag: No tag Purity: > 95% by Tris-Bis PAGE;> 95% by SEC-HPLC Endotoxin: preparation: 0.22 m filtered solution of PBS, pH 7.4. Storage Aliquot and store at -80C. Avoid repeated freeze/thaw cycles.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: 0.2 mg/mL in sterile PBS, pH7.4Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : CCL2, also called monocyte chemotactic protein?1 (MCP?1) or JE, is a member of the C?C or beta chemokine family that is best known as a chemotactic agent for mononuclear cells (1, 2). Human CCL2 cDNA encodes a 99 amino acid (aa) precursor protein with a 23 aa signal peptide and a 76 aa mature protein (2). Removal of the first 5 aa of the mature protein, including the N-terminal pyrrolidone carboxylic acid?modified glutamine, occurs naturally by metalloproteinase cleavage and down?regulates activity but not receptor binding (3). CCL2 may form multiple bands from 8.7?13.5 kDa on SDS?PAGE due to non?covalent dimerization and variable carbohydrate content (3). Mature human CCL2 shares 78?79% aa identity with canine, porcine and equine CCL2, while mouse and rat express a form of CCL2 that is extended by 49 aa and shares only ~56% aa identity within the common region. Human CCL2 can, however, induce a response in murine cells (4). Fibroblasts, glioma cells, smooth muscle cells, endothelial cells, lymphocytes and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation, but monocytes and macrophages appear to be the major source (1, 2). In addition to its chemotactic activity, CCL2 induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils that express its receptor, CCR2 (2). Additionally, it promotes Th2 polarization in CD4+ T cells (5). CCL2?mediated recruitment of monocytes to sites of inflammation is proposed to play a role in the pathology of atherosclerosis, multiple sclerosis and allergic asthma (6, 7). GeneID 6347 Swiss Prot P13500 Alias: CCL2;GDCF-2;HC11;HSMCR30;MCAF;MCP-1;MCP1;SCYA2;SMC-CFMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
Kappa-Casein Protein
IFN-gamma Protein
Popular categories:
CD121b/IL-1 Receptor 2