Species: Human Expressing host: HEK293 cells Description: Recombinant Human Hepatocyte growth factor/HGF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln32-Ser728) of human Hepatocyte growth factor/HGF (Accession #NP_000592.3) fused with and a 6His tag at the C-terminus. Tag: C-His Purity: > 95% by SDS-PAGE. Endotoxin: Biological Activity: Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50for this effect is 2.23-8.91 ng/mL, corresponding to a specific activity of 1.12105-4.48105units/mg. preparation: Lyophilized from a 0.22 m filtered solution of PBS, pH 7.4. Storage Store the lyophilized protein at -20C to -80C for 12 months.
After reconstitution, the protein solution is stable at -20C for 3 months, at 2-8C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenicfactor. It belongs to the peptidase S1 family and Plasminogen subfamily, contains 4 kringle domains, 1 PANdomain and 1 peptidase S1 domain. HGF regulates cell growth, cell motility, and morphogenesis by activatinga tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF is secreted bymesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability tostimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis,and tissue regeneration. GeneID 3082 Swiss Prot P14210 Alias: HGF;DFNB39;F-TCF;HGFB;HPTA;SFMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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