Species: Human Expressing host: E. coli Description: Recombinant Human TNFSF13B/BAFF/CD257 Protein is produced byE. coliexpression system. The target protein is expressed with sequence ( Ala134-Leu285) of human TNFSF13B (Accession #NP_006564.1). Tag: No tag Purity: > 90% by SDS-PAGE. Endotoxin: Please contact us for more information. Biological Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized recombinant human BAFF at 5 g/mL (100 L/well) can bind recombinant human BCMA with a linear range of 3-20 ng/mL.
2.Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50for this effect is 0.1-1 ng/mL, corresponding to a specific activity of 1106-1107units/mg.
3.Loaded Human TNFRSF17/BCMA/CD269 Protein, C-hFc&His (Catalog: RP00155) on ProA Biosensor, can bind Human TNFSF13B/BAFF/CD257 Protein, no Tag (Catalog: RP00018) with an affinity constant of 3.05nM as determined in BLI assay (Gator). preparation: Lyophilized from a 0.22 m filtered solution of 50mM Tris, 150mM NaCl, pH 8.0.Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : The protein is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. GeneID 10673 Swiss Prot Q9Y275 Alias: TNFSF13B;BAFF;BLYS;CD257;DTL;TALL-1;TALL1;THANK;TNFSF20;TNLG7A;ZTNF4MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
CD38 Protein
PF-4/CXCL4 Protein
Popular categories:
Alpha 2 Antiplasmin