Species: Human Expressing host: HEK293 cells Description: Active Recombinant Human TNFSF11/RANKL/CD254 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly136-Asp317) of human TRANCE/RANK L/TNFSF11 (Accession #NP_143026.1) fused with a 6His tag at the N-terminus. Tag: N-His Purity: > 90% by SDS-PAGE. Endotoxin: Biological Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF11 Protein at 2 g/mL (100 L/well) can bind Mouse TNFRSF11B with a linear range of 0.049-13.74 ng/mL.
2.Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 10.85-43.42 ng/mL, corresponding to a specific activity of 2.30104~9.22104 units/mg. preparation: Lyophilized from a 0.22 m filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : Tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is a single-pass type II membrane protein which belongs to the tumor necrosis factor family. TNFSF11 is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. TNFSF11 was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. GeneID 8600 Swiss Prot O14788 Alias: TNFSF11;CD254;ODF;OPGL;OPTB2;RANKLMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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