Species: Human Expressing host: E. coli Description: Recombinant Human/Mouse/Rat BMP-4 Protein is produced by Escherichia coli expression system. The target protein is expressed with sequence of Human BMP-4 fused with polyhistidine tag at the C-terminus Tag: C-His Purity: > 95% by SDS-PAGE. Endotoxin: Biological Activity: Measured by its ability to inhibit alkaline phosphatase production by ATDC5 cells. The ED50for this effect is preparation: Lyophilized from a 0.22 m filtered solution of 20 mM sodium carbonate, pH 9.0. Storage Store the lyophilized protein at -20C to -80C for long term.
After reconstitution, the protein solution is stable at -20C for 3 months, at 2-8C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : Bone morphogenetic protein 4 is a protein that in humans is encoded by BMP4 gene. BMP4 is found on chromosome 14q22-q23. BMP4 is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. BMP4 is highly conserved evolutionarily. BMP4 is found in early embryonic development in the ventral marginal zone and in the eye, heart blood and otic vesicle. GeneID 652 Swiss Prot P12644 Alias: BMP2B; BMP2B1; MCOPS6; OFC11; ZYME;BMP4;BMP2B1;MCOPS6;OFC11;ZYMEMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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