Species: Human Expressing host: HEK293 cells Description: Recombinant Human PECAM-1/CD31 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln28-Lys601) of human CD31/PECAM1 (Accession #NP_000433.4) fused with a 6His tag at the C-terminus. Tag: C-His Purity: > 90% by SDS-PAGE. Endotoxin: Biological Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human CD31 at 1 g/mL (100 L/well) can bind Human CTNNB1 with a linear range of 9.7 ng/mL-1.5 g/mL.2.Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8104 cells/well are added to CD31 coated plates (5g/mL, 100 L/well) in the presence of 10 g/mL PHA,approximately 60-70% will adhere after 30 minutes at 37.
3.Measured by its binding ability in a functional ELISA.Immobilized APC anti-human CD31 Antibody at 1g/mL (100 L/well) can bind Human CD31 Protein with a linear range of 4-25.8 ng/mL. preparation: Lyophilized from a 0.22 m filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. GeneID 5175 Swiss Prot P16284 Alias: C85791; Cd31; PECAM-1; Pecam;PECAM1;CD31; PECA1; GPIIA’; endoCAM; CD31/EndoCAM;CD31/EndoCAM;PECA1;PECAM-1;endoCAM;GPIIAMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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