Species: Human Expressing host: E. coli Description: Recombinant Human MANF Protein is produced byE. coliexpression system. The target protein is expressed with sequence (Leu25-Leu182) of human MANF (Accession #NP_006001.4) fused with an initial Met at the N-terminus and a 6His tag at the C-terminus. Tag: C-His Purity: > 92% by SDS-PAGE. Endotoxin: preparation: Lyophilized from a 0.22 m filtered solution of 20mM PIPES, 500mM NaCl, pH 6.0.Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : The protein is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing this protein increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. GeneID 7873 Swiss Prot P55145 Alias: MANF;ARMET;ARPMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
PRSS3/Trypsin-3 Protein
PLK1 Protein
Popular categories:
ACP5
ALCAM/CD166