Species: Human Expressing host: E. coli Description: Recombinant Human Somatotropin/GH-N/GH1 Protein is produced byE. coliexpression system. The target protein is expressed with sequence (Phe27-Phe217) of human GH (Accession #NP_000506.2) fused with an initial Met at the N-terminus and a 6His tag at the C-terminus. Tag: C-His Purity: > 92% by SDS-PAGE. Endotoxin: Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human GH1 at 2 g/mL (100 L/well) can bind Human GHR with a linear range of 0.1-19.4 ng/mL. preparation: Lyophilized from a 0.22 m filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin. It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretary granules. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones – GH-releasing hormone (GHRH) and somatostatin (SST) – as well as by signals from the periphery – ghrelin and leptin. Human GH is a pleiotropic cytokine that exerts its biological actions by binding to the transmembrane GH receptor, which is present in many cell types. GH stimulates the liver and other tissues to produce IGF-1, which regulates growth and metabolism. GH has also been shown to have direct effects on growth that is independent of IGF-1. GH, directly or indirectly via IGF-1, can act on B cells, T cells, NK cells, macrophages and neutrophils to exert immunomodulatory activities. In addition, GH can act directly on various cell types to induce lipolysis, lactation, amino acid uptake and protein synthesis. GeneID 2688 Swiss Prot P01241 Alias: GH1; GH; GH-N; GHB5; GHN; IGHD1B; hGH-N; somatotropin;GH;GH-N;GHB5;GHN;IGHD1B;hGH-NMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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