Species: Human Expressing host: Pichia Description: Recombinant Human IGF-I Protein is produced byPichiaexpression system. The target protein is expressed with sequence (Gly49-Ala118) of human IGF-I (Accession #NP_001104755.1) fused with no additional amino acid. Tag: No tag Purity: > 95% by SDS-PAGE. Endotoxin: Biological Activity: Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50for this effect is typically 53.5-214.2 ng/mL. preparation: Lyophilized from a 0.22 m filtered solution of PBS, pH 7.4. Storage Store the lyophilized protein at -20C to -80C for long term.
After reconstitution, the protein solution is stable at -20C for 3 months, at 2-8C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : GeneID 3479 Swiss Prot P05019-1 Alias: IGF-1;IGF-I;IGF1A;IGFI;IGF?I;MGF;IGF1MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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