Species: Human Expressing host: HEK293 cells Description: Recombinant Human VEGF-A/VEGF165 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala27-Arg191) of human VEGF165 (Accession #NP_001165097.1) fused with a 6His tag at the N-terminus. Tag: N-His Purity: > 97% by SDS-PAGE. Endotoxin: Biological Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human VEGF165 at 1 g/mL (100 L/well) can bind Recombinant Human VEGFR2 with a linear range of 8-20 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human VEGF165 at 2 g/mL (100 L/well) can bind Human KDR with a linear range of 0.2-11.6 ng/mL. preparation: Lyophilized from a 0.22 m filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : GeneID 7422 Swiss Prot P15692-4 Alias: VEGFA; MVCD1; VEGF; VPF; vascular endothelial growth factor A;MVCD1;VEGF;VPF;L VEGFA;VEGF AMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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