Species: Human Expressing host: E. coli Description: Recombinant Human IL-1 beta Protein is produced byE. coliexpression system. The target protein is expressed with sequence (Ala117-Ser269) of human IL-1 beta (Accession #NP_000567.1) fused with an initial Met at the N-terminus and a 6His tag at the C-terminus. Tag: C-His Purity: > 95% by SDS-PAGE. Endotoxin: Biological Activity: 1.Recombinant Human IL1 protein was measured by NF-B reporter assay in HEK293 cells. The ED50for this effect is 1-10 pg/mL, corresponding to a specific activity of 1108~1109units/mg.
2.Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50for this effect is 0.217-0.870 ng/mL, corresponding to a specific activity of 1.15106~4.61106units/mg.
3.Measured by its binding ability in a functional ELISA. Immobilized Human IL-1 beta (Catalog: RP00002) at 10 g/mL (100 L/well) can bind Biotinylated Human IL-1R1 (Catalog: RP01036) with a linear range of 0.002-1 g/mL. preparation: Lyophilized from a 0.22 m filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family.This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. GeneID 3553 Swiss Prot P01584 Alias: IL-1;IL1-BETA;IL1F2;IL1 beta;IL1BMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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