Product: BM5532PBromodeoxyuridine / BrDU antibody
Quantity: 0.1 mg
Synonyms:
Presentation: Purified
Clonality: Monoclonal
Host: Mouse
Isotype: IgG2a
CAS NO: 4759-48-2 Product: Isotretinoin
Shipping to: Worldwide
Immunogen: Bromodeoxyuridine
Application: Detection of Bromodeoxyuridine incorporation in tissue and cell culture. (See protocols on page 2.Working Dilution: 1/10.Incubation time: 30 min at RT.
Background: Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside which is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of repli
Concentration:
Storage: Prior to reconstitution store at 2-8°C.Following reconstitution store the antibody -20°C.Avoid repeated freezing and thawing.Shelf life: one year from despatch.
Buffer System: PBS buffer, pH 7.4 containing 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer
Preservatives:
State: Lyophilized purified IgG fractionPurified
Specifictiy: The antibody is specific for Bromodesoxyuridine and does not cross-react with Thymidine. It is a valuable tool for detection of S-phase (DNA-synthesizing cells) in cell ad tissue culture. 3H-thymidine labelling techniques can be avoided.