Product: BM6048PBromodeoxyuridine / BrDU antibody
Quantity: 0.1 mg
Synonyms:
Presentation: Purified
Clonality: Monoclonal
Host: Mouse
Isotype: IgG1
CAS NO: 60282-87-3 Product: Gestodene
Shipping to: Worldwide
Immunogen: IIB5 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse intraperitoneally immunized with BrdU conjugated to BSA
Application: Flow Cytometry, Immunocytochemistry. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded tissues.Recommended Dilutions: 1/100-1/200 for Flow Cytometry, and for Immunohistochemistry with avidin-biotinylated horseradish peroxi
Background: The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent
Concentration: 1.0 mg/ml
Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freeze-thaw cycles.Shelf life: One year from despatch.
Buffer System: PBS
Preservatives: 0.09% Sodium Azide
State: Liquid purified IgG fractionPurified
Specifictiy: IIB5 reacts with Bromodeoxyuridine (BrdU) also when incorporated into nuclear DNA. The antibody is known to cross-react with Iododeoxyuridine (IdU). Although we have no specific information concerning Chlorodeoxyuridine (CldU), it is to be expected that a