Species: Human Expressing host: HEK293 cells Description: Recombinant Human 5′-Nucleotidase/CD73 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Lys547) of human 5′-Nucleotidase/CD73 (Accession #NP_002517.1) fused with a 6His tag at the C-terminus. Tag: C-His Purity: > 95% by SDS-PAGE. Endotoxin: Biological Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human 5-Nucleotidase/CD73 at 2 g/mL (100 L/well) can bind 5-Nucleotidase/CD73 Rabbit pAb with a linear range of 0.16-2.93 ng/mL.
2.Measured by its ability to hydrolyze the 5-phosphate group from the substrate adenosine-5-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit. The specific activity is >33654 pmol/min/g.
3.Measured by its ability to hydrolyze the 5-phosphate group from the substrate adenosine-5-monophosphate (AMP). The specific activity is >25,000 pmol/min/g. preparation: Lyophilized from a 0.22 m filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : 73, an ecto-5′-Nucleotidase, is an ectoenzyme expressed by most cell types. CD73 is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The proteins are used as determinants of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. GeneID 4907 Swiss Prot P21589 Alias: NT5E;CALJA;CD73;E5NT;NT;NT5;NTMedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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