Purity: > 97 % as determined by SDS-PAGE Endotoxin: Biological Activity: Verified Protein construction: A DNA sequence encoding the extracellular domain (Met 1-Pro 345) of mouse CD14 (NP_033971.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. NP No. NP_033971.1 Expressing host: HEK293 Cells Species: Mouse Ser 16 Molecular Weight: The recombinant mouse CD14/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 578 amino acids and has a predicted molecular mass of 63.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD14/Fc monomer is approximately 85-95 kDa due to glycosylation. Buffer solution: Lyophilized from sterile PBS, pH 7.4
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Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. Transportation method: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. stability & Storage: Samples are stable for up to twelve months from date of receipt at -20 to -80. Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Redissolution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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