Species: Human Expressing host: HEK293 Description: Recombinant human IL-27A&IL-27B Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Arg21-Lys229&Ile23-Ser328) of human IL-27A&IL-27B (Accession #) fused with addtional amino acid free. Tag: No tag Purity: > 95% by Tris-Bis PAGE;> 95% by SEC-HPLC Endotoxin: preparation: 0.22 m filtered solution of PBS, pH 7.4. Storage Aliquot and store at -80C. Avoid repeated freeze/thaw cycles.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: 0.2 mg/mL in sterile PBS, pH7.4Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV-induced gene?3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the?28 kDa glycoprotein that is related to the p35 chain of IL-12 (2-4). The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein (5). The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in the hematopoietin receptor family (5). Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. Human EBI3 is 61% aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215?aa mature region (6). GeneID 246778&10148 Swiss Prot Q8NEV9&Q14213 Alias: Human IL-27; h-IL-27; rh-IL-27; recombinant IL-27; interleukin-27MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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