Species: Human Expressing host: E. coli Description: Recombinant Human FGF-1/aFGF Protein is produced byE. coliexpression system. The target protein is expressed with sequence (Phe16-Asp155) of human FGF acidic/FGF1 (Accession #NP_000791.1) fused with a 6His tag at the C-terminus. Tag: C-His Purity: > 95% by SDS-PAGE. Endotoxin: Biological Activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human FGF1 at 5 g/mL (100 L/well) can bind Recombinant Human FGFR3 with a linear range of 0.5-1.5 g/mL.
2.Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50for this effect is typically 22-140 pg/mL. preparation: Lyophilized from a 0.22 m filtered solution of 50mM MOPS,100mM Na2SO4,10mM EDTA, pH7.9Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : GeneID 2246 Swiss Prot P05230-1 Alias: AFGF; ECGF; ECGF-beta; ECGFA; ECGFB; FGF-1; FGF-alpha; FGFA; GLIO703; HBGF-1; HBGF1;FGF1;ECGF;ECGF-beta;ECGFA;ECGFB;FGF-1;FGF-alpha;FGFA;GLIO703;HBGF-1;HBGF1MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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