Species: Human Expressing host: E. coli Description: Recombinant Human Neurotrophin-3/NGF-2/NT-3 Protein is produced byE. coliexpression system. The target protein is expressed with sequence (Tyr139-Thr257) of human NT-3/Neurotrophin-3 (Accession #P20783). Tag: No tag Purity: > 95% by SDS-PAGE. Endotoxin: preparation: Lyophilized from a 0.2 m filtered solution of 20mM PB, 250mM NaCl, pH 7.2.Contact us for customized product form or formulation. Storage Store the lyophilized protein at -20C to -80 C for long term.
After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week.Unopened freeze-dried powder,Store at -20 to -80 °C for twelve months
Redissolution: Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.Redissolution:Store at -20 to -80 °C,avoid repeated freeze thaw cycles
Background Information : Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors and is structurally related to -NGF, BDNF and NT-4. The NT3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptideand a proprotein that are cleaved to yield the 119 amino acid residue mature NT3.The amino acid sequencesof mature human, murine and rat NT-3 are identical. NT-3 selectively promotes the differentiation and survivalof specific neuronal subpopulations in both the central as well as the peripheral nervous systems. GeneID 4908 Swiss Prot P20783 Alias: NTF3;HDNF;NGF-2;NGF2;NT-3;NT3MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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